This new decide to try is leftover within minus 80 °C up to DNA extraction
Sampling and you will DNA removal
An example away from leg muscle tissues is extracted from a-dead bird grabbed of the police during the Sihong State, Asia, towards the , zero traditions creature was working in this research. I removed genomic DNA regarding tissue utilising the Common Genomic DNA Extraction Equipment (Takara Biomedical Technology Co. Ltd., Beijing, China). The fresh new DNA high quality try measured having fun with agarose gel electrophoresis. DNA purity and you may focus have been reviewed because of the NanoDrop 2000 (NanoDrop Development, Wilmington, NC, USA).
Mitogenome sequencing and you will set-up
After the DNA removal, i fragmented step 1 ?g regarding purified DNA and you will used it in order to created two hundred bp small-submit libraries. These qualified libraries was in fact sequenced having PE150 bp into the an enthusiastic BGISEQ-500 sequencer according to maker’s rules detail by detail in the earlier literature 40 . Consequently, i gotten 5.dos Gb away from clean data and you will trimmed all intense reads playing with FASTQ 41 . Next, i mapped this new highest-top quality reads using standard parameters so you’re able to reference Aythya mitogenome datasets (accession quantity: AF090337, KJ710708, KJ722069), hence i taken from GenBank courtesy Bowtie 42 .